Part:BBa_J119052:Design

J23100 promoter for BsaI GGA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 18
  Illegal NheI site found at 41
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 52
  

Design Notes

The 5' word is CGAC and the 3' word is GCGG. The part can be assembled into J119023 using these words.

Source

de novo DNA synthesis